Plasmid_Backbone

Part:BBa_K1223009:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-09)


pUC57 backbone (REVERSED)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 25
    Illegal PstI site found at 2678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1341
    Illegal SapI.rc site found at 2423


Design Notes

cloning of P.A.S.E. 1&2 cassettes.


Source

genebank

References